Screening of Amazonian bacillus strains for lipase production using glycerol as substrate


  • Giandra Volpato Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul
  • Victória Furtado Migliavacca Universidade Estadual do Rio Grande do Sul
  • Bruna Coelho de Andrade Pontifícia Universidade Católica do Rio Grande do Sul
  • Júlio Xandro Heck Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul
  • Marco Antônio Záchia Ayub Universidade Federal do Rio Grande do Sul



The industrial application of lipolytic enzymes has been studied mainly due to the ability of these enzymes in catalyze reactions of synthesis and their stability in various organic solvents. One possibility is the use of lipase the organic synthesis, taking advantage as the generation of waste and difficult recovery of sub bioproducts. In this work, we carried out a selection of eighty-four isolates of Bacillus amazonian for lipase production, of which 30 strains showed lipolytic activity. The study of the culture conditions was performed through a Plackett-Burman experimental design using the strain that presented the highest lipolytic activity in a culture medium using glycerol as substrate.  The studied conditions were: concentration of soybean oil, olive oil, triton X-100, gum arabic, glycerol, and (NH4)2SO4, pH, temperature and concentration of inoculums. The best result obtained were 27 U/L in 48 h of cultivation by Bacillus circulans BL53. This work shows that the search and selection of microorganism with lipolytic activities can facilitate the discovery of new lipases, with potential use as by-product surplus.